The current study investigated the anti-herpetic activity of S. purpurea in HSV-1 infected Vero cells. The results presented also support that the S. purpurea extract inhibited replication of HSV-1 at a point following viral uptake into the host cell. Oral Radiol. At 8h.p.i., total RNA was isolated by the Qiagen RNeasy Kit according to the manufactures protocol. Pitcher plant contains tannins and other chemicals that are thought to help with some digestive tract problems. Following incubation, the virus was pelleted by centrifugation at 20,000g for 1h, washed with media, and resuspended in complete media. Pitcher plant is often sold as an herbal supplement. Physician's Desk Reference. Montvale: Medical Economics 2000. Herpes simplex virus type-1 (HSV-1), one of the most widely spread human viruses in the Herpesviridae family, causes herpes labialis (cold sores) and keratitis (inflammation of the cornea). 2001 Oct 19;294(5542):500. doi: 10.1126/science.294.5542.500. You are using a browser version with limited support for CSS. ADS In this study, we highlight and characterize of the anti-herpetic activity of the carnivorous plant, S. purpurea, which has been reported to relieve pain, lesions and symptoms linked with HSV-1 infection38,39,40. Kim, N. S., Jeong, S. I., Hwang, B. S., Lee, Y. E. & Kang, S. H. Gallic acid inhibits cell viability and induces apoptosis in human monocytic cell line U937. Miles HC. Mishra, K. P., Sharma, N., Diwaker, D., Ganju, L. & Singh, S. B. Bioterrorism. Li, D., Wang, P. Q. In the nineteenth century, smallpox ravaged through the United States and Canada. J. Virol. Although, natural smallpox no longer poses a health threat, there is a remote possibility thatunstable states or terrorist groups could have acquiredstocks of the virusfollowing the collapse of the Soviet Union, which had developed smallpox as a biological warfare agent. J. S. purpurea temporal inhibition of HSV-1 replication. Fleming T, ed. You're not signed in. Pitcher plant is taken by mouth for digestive disorders, particularly constipation; for urinary tract diseases and fluid retention; as a cure for smallpox; and to prevent scar formation. All the experiments performed in Fig. Cite this article. An infusion of the leaves was at one time considered to be a cure for smallpox[4, 257], Arizona State University reached a positive outcome testing Saracenia Purpurea vs. smallpox . CAS Dermatol. Gene expression levels were normalized to actin. Google Scholar. Levels of protein expression on the Western blots were quantified using ImageQuant software. Cellular GAPDH was used as an internal reference and normalization. Vitamin D Deficiency: How Much Vitamin D Is Enough? Biol. S. purpurea inhibited HSV-1 ICP4, ICP8, and gC gene expression. Current therapeutic drugs, such as acyclovir and its derivatives, have been used in the treatment of HSV-1 infection, however, these drugs are expensive and can result in viral resistance in patients with AIDS and patients with drug-induced immunosuppression. http://www.henriettesherbal.com/eclectic/spec-med/sarracenia.html, R01 AI095394/AI/NIAID NIH HHS/United States. 207, 12951305 (2013). MATH Honess, R. W. & Roizman, B. Google Scholar. Access this article for 1 day for:30 / $37 / 33 (excludes VAT). With the renewed threat of poxvirus-related infections, our results indicate Sarracenia purpurea may act as another defensive measure against Orthopoxvirus infections. 2, 2 (2013). Thank you for visiting nature.com. The experiments of Dr. Porcher, of South Carolina, showed that it exerted a marked influence on the sympathetic. Figure 5. Antiviral Potential of Melissa officinalis L.: A Literature Review. Notably, the titers of HSV-1 when treated at 1, 4, and 6h.p.i. https://doi.org/10.1371/journal.pone.0140765 (2015). This website collects cookies to deliver a better user experience. 48, 199226 (1994). Perng, G. & Jones, G. Towards an understanding of the herpes simplex virus type 1 latency-reactivation cycle. Follow all directions on the product label and package. We use a state-of-the-art microprocessor. J.L. The plant/liquid mixture was centrifuged at 3000g for 15min and the supernatant filtered through a 0.2m syringe filter. Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis. Anti-herpes virus activity of the carnivorous botanical, https://doi.org/10.1038/s41598-020-76151-w. Get the most important science stories of the day, free in your inbox. Vero cells were infected with 100200 (plaque forming units) pfu of HSV-1 KOS in the presence of increasing concentrations of S. purpurea or vehicle (50% ethanol, 10% glycerin) for 1h at 37C followed by incubation in media containing S. purpurea or vehicle (50% ethanol, 10% glycerin) for 3days at 37C. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Please note: your email address is provided to the journal, which may use this information for marketing purposes. & Schnitzler, P. Melissa officinalis extract inhibits attachment of herpes simplex virus in vitro. Adults: 4 drops into a tsp. Insects are attracted into the lurid red or purple pitchers, and are then prevented from getting out by downward-pointing hairs. Oral. If you choose to use pitcher plant, use it as directed on the package or as directed by your doctor, pharmacist, or other healthcare provider. Spear, P. G., Shieh, M. T., Herold, B. C., WuDunn, D. & Koshy, T. I. Heparan sulfate glycosaminoglycans as primary cell surface receptors for herpes simplex virus. The effect of S. purpurea extracts on VACV replication. L.K., As.K., Ar.K. To test for this, Vero cells were infected with HSV-1, treated with S. purpurea extracts at 0, 1, 2, 4, and 6h.p.i., followed by purification of the RNA at 8h.p.i. Extracts from the carnivorous pitcher plant, Sarracenia purpurea, have previously been shown to inhibit the replication of HSV-1. compared to treatment at 1, 4, and 6h.p.i. Nakabayashi, J. The late proteins form the capsid and the receptors on the surface of the virus. Noormohamed, F. H., Youle, M. S., Higgs, C. J., Martin-Munley, S. & Gazzard, B. G. Pharmacokinetics and absolute bioavailability of oral foscarnet in human immunodeficiency virus-seropositive patients. Dauber, B., Saffran, H. A. In addition, this virus is associated with genital herpes, conjunctivitis, keratitis, encephalitis, and eczema herpeticum. At this time, a botanical preparation, derived from the carnivorous plant Sarracenia purpurea, was proclaimed as being a successful therapy for smallpox infections. Information not dated. Seek emergency medical attention or call the Poison Help line at 1-800-222-1222. Global and regional estimates of prevalent and incident herpes simplex virus type 1 infections in 2012. The work described characterizes the antipoxvirus activity associated with this botanical extract against vaccinia virus, monkeypox virus and variola virus, the causative agent of . For the cells receiving multiple S. purpurea treatments, media was replaced with fresh media containing the varying amounts of S. purpurea extract every six hours. In vitro efficacy of brincidofovir against variola virus. Sarracenia purpurea has few reliable indications, but many homeopaths report great success in severe cases. Phytother. Pitcher plant taken by mouth has been used in alternative medicine to treat constipation, urinary tract problems, digestion problems, fluid retention, and other conditions. Input virus was harvested at 1h.p.i. Botanical extract The following herbs were used in this study: Sarracenia purpurea, Astragalus membranaceus, Echinacea angustifolia, and Coriolus versicolor. Smith, J. S. & Robinson, N. J. Age-specific prevalence of infection with herpes simplex virus types 2 and 1: A global review. (detailed description of each of the ratings). In the current study, we demonstrate that S. purpurea extracts can inhibit the replication of HSV-1 through two distinct mechanisms of action. Chen, T. et al. J. Infect. 14, 819 (1974). 2021 Aug 11;29(8):1266-1276.e5. Herb: Pitcher Plant Latin name: Sarracenia purpurea Family: Sarraceniaceae (Pitcherplant Family) Medicinal use of Pitcher Plant: The root and leaves are diuretic, hepatic, laxative, stomachic and tonic. Parker S, Chen NG, Foster S, Hartzler H, Hembrador E, Hruby D, Jordan R, Lanier R, Painter G, Painter W, Sagartz JE, Schriewer J, Mark Buller R. Antiviral Res. PLoS ONE 8, e62482 (2013). Since previous work using S. purpurea extracts against poxviruses demonstrated that the extract did not disrupt the poxvirus envelope34, we propose that the extract is likely blocking HSV-1 attachment to the cell, although further studies to confirm this are required. 180 Years. 105, 5563 (2006). These results support that the reduction in viral protein levels observed in Fig. The appropriate dose of pitcher plant depends on several factors such as the user's age, health, and several other conditions. At 24h.p.i, virus was harvested and titered. Drugs. (Fig. by scraping into the media. j. to gtts xx. 5). Looker, K. J. Phytochemistry 94, 238242 (2013). Google Scholar. HHS Vulnerability Disclosure, Help Sarapin is a grandfathered FDA-approved prescription product. The monolayers were washed three times to remove the S. purpurea extract. SP-gel: aqueous extract of Sarracenia purpurea in gel base, VG: Vehicle gel control, HSV: herpes simplex virus. The immediate-early genes are typically involved in controlling host cell function, for example, ICP4 plays a significant role in the inhibition of host gene transcription. PubMed All plant material was subsequently verified by qualified botanical specialists using reference keys. J. Virol. A pitcher plant extract (Sarapin) is given as a shot. Copyright 2023 BMJ Publishing Group Ltd, Treatment of Small-Pox by Sarracenia Purpurea, Birmingham and Solihull Mental Health NHS Foundation Trust: Consultant Psychiatrist General Adult - Northcroft CMHT, Brent Area Medical Centre: Salaried GP - Brent Area Medical Centre, Onebright Ltd: Consultant Psychiatrist (Neurodiversity) - Remote / London, The Royal Hospital for Neurodisability: Clinical Fellow, Womens, childrens & adolescents health. provided experimental design and interpretation. Garner, J. 264, 74057411 (1989). Microbiol. It is not known whether pitcher plant passes into breast milk or if it could harm a nursing baby. Drugs like foscarnet, a pyrophosphate analog, and cidofovir, a nucleotide analog, can be used when acyclovir-resistance has developed, although these drugs display reduced bioavailability and nephrotoxicity, respectively11,12,13,14. https://doi.org/10.1038/s41598-020-76151-w, DOI: https://doi.org/10.1038/s41598-020-76151-w. Anti-herpes virus activity of the carnivorous botanical, Sarracenia purpurea. The site is secure. Medically Documented. Often used as specific, as well as prophylactically (for more details about this remedy, see below). may suggest the S. purpurea extract can inhibit HSV-1 replication at two distinct steps in the viral replication process. Competing Interests: Yvan Rochon, an author on the submitted manuscript, is owner and operator of Herbal Vitality, Inc. Dose from gtts. Lancet 80, 430431 (1862). 2012 Apr;94(1):44-53. doi: 10.1016/j.antiviral.2012.02.005. Selected plant species from the Cree pharmacopoeia of northern Quebec possess anti-diabetic potential. and transmitted securely. RT-PCR was done to determine the levels of HSV-1 ICP4, ICP8, and gC genes and normalized to cellular GAPDH. When Vero cells were treated with S. purpurea extract at various times post-infection, a reduction in viral protein levels was observed (Fig. Antimicrob. Treatment of herpes virus-associated lesions using a synergistic botanical blend. J. Nicola, A. V. & Straus, S. E. Cellular and viral requirements for rapid endocytic entry of herpes simplex virus. To quantitate this anti-HSV-1 effect, a plaque reduction assay was performed. The virus is highly prevalent and endemic worldwide. Repeat at greater intervals as condition subsides. All Natural! Slider with three articles shown per slide. J. Physiol. To examine this further, free HSV-1 virions were incubated with the extract, followed by washing of the virus and subsequent infection. PubMed To further confirm the anti-HSV-1 activity of S. purpurea, a single-step growth curve experiment was performed. 1A, HSV-1 infection induced observable CPE after 24h. When virally infected cells were treated with increasing doses of the extract, this CPE was moderately to fully inhibited (Fig. The active constituent(s) in M. officinalis is caffeic acid and/or its derivatives58.

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